I am observing binding of my drug to regenerated cellulose dialysis membrane (from Thermofisher). Is there a way to coat the membrane to prevent nonspecific binding? or an alternate source of membrane would help too.
Thx
Manish
Often the presence of a higher concentration component to take up binding sites can compete and help...sites can then get saturated... I've seen this with in vivo microdialysis probes in lab animals, but your application may be far different. Size and hydrophobicity both make a big difference. Likewise, your own compound might well saturate membrane if there is enough of it present...
BSA has worked for us in some cases as long as the small molecule we were studying did not bind stongly to BSA itself....this can be a good approach as Neil suggests.
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