Ahoy,
I would like to fix Zebrafish embryos that require FACS to isolate eGFP expressing germ-line cells at a later stage, followed by DNA extraction.
I imagine it should be possible to collect the embryos, dissociate them using force+trypsine, then dapi stain them and then PFA fix them before freezing them until FACS. However, the PFA will make it tricky to extract DNA.
Has anyone done this or knows of a protocol?
It might be possible for me not to fix them and FACS them right away but if I can fix them that would make everything a lot easier, more efficient and even cheaper.
Thank you!
Tim
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