Tim Moser

3 Questions 4 Answers 0 Followers

Questions related from Tim Moser

SPRI-Bead DNA extraction of Juvenile Zebrafish - Is it eumelanin and how to I get rid of it?

Ahoy, I am performing SPRI-Bead extraction of 29 day old (whole) Zebrafish. The euthanized and frozen fish are digested for 2-5h in 20mg/ml Proteinase-K in TNES buffer. This leaves me; depending...

31 January 2020 1,142 1 View

Is there a way to perform FACS and DNA extraction on fixed eGFP cells?

Ahoy, I would like to fix Zebrafish embryos that require FACS to isolate eGFP expressing germ-line cells at a later stage, followed by DNA extraction. I imagine it should be possible to collect...

01 August 2019 7,025 1 View

Tricks for easier Zebrafish egg/embryo sorting.

Ahoy, Firstly, sorry for staying somewhat vague but I am not allowed to go into more details about the drug its effects and the wanted outcome. I am currently conduction an experiment where I...

01 January 1970 4,820 2 View