I am trying to quantify the periodicity and randomness of a sarcomeric structure in muscle. For this I used ImageJ to convert my images of sarcomere protein stain to FFT. This is a 2D FFT. I would like to get the 1D information out from these images to get the frequency and amplitude info from the FFT images. Does someone have experience in Fourier Transformation (other than using it to remove noise from an image).?