The NCBI Entrez E-utiliies documentation describes how to download genome annotations in GenBank format via their API. But is there a way to download a genome annotation in GFF3 format in an automatic manner?
Try FTP site.
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.
25 July 2024 9,976 3 View
I have seen two kinds of explanations given for the phenomenon that I try to draw in the attached png document. One explanation says that an air bubble creates the experience of a solid black...
16 June 2024 6,493 1 View
Cross-slip, twinning and fracture are major deformation modes adopted by loaded materials. It appears sound that these apparently different deformation mechanisms can be analysed on the equal manner!
15 June 2024 8,477 3 View
The terms Fictional Language, Fictitious Language, Artificial Language, or Constructed Language in fiction has been used interchangeably in papers that I have read. Are there differences in these...
05 June 2024 3,453 7 View
Investigating use of Sorghum as part of Regenerative Agriculture management system using cover crops & mower-crimping, Alley Cropping, sheep manure in a zero till management system with...
18 April 2024 2,389 3 View
I'm conducting a multiple case study, and I'm interested in exploring a phenomenon from the perspectives of male and female participants.
14 April 2024 4,352 1 View
I need to prepare Na2HPO4/Citrate buffer pH 5.0, it is possible to prepare 1M, 0r 0.5M if yes, please how? Thank you I want to crystallize my protein with this buffer? i need 0.1M working...
03 April 2024 10,006 0 View
The following are the details of the conference: Name of event: International Conference on Community Health Nursing and Public Health (ICCHNPH) Venue: Brno, Czech Republic Date: 9th -10th...
25 March 2024 996 1 View
For cell culture , if want to study about the protein or gene expression ? The treatment need to dilute with medium without fbs & pen strep or complete medium. What happen , if the treatment...
19 March 2024 399 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,339 2 View
Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...
04 July 2024 9,875 1 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
Dear Sir, I hope this message finds you well and in good health. I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master....
30 June 2024 8,650 0 View
Hi, everyone I would like to know if there is there any bioinformatic tool that can download batch DNA seq using imported NCBI accession numbers (upto 100 sequences) and translate them into...
19 June 2024 1,917 2 View
I am working on a paper for my bioinformatics class, where we are running an analysis of transcriptomics on A. thaliana col-0 about the difference in gene expression in the temperatures of 21 and...
12 June 2024 8,822 1 View
Hi there, I have some 16S and metagenomic sequencing data from mice fecals and I want to upload them to NCBI Sequence Read Archive (SRA). I don't know what NCBI package is suitable for my animal...
03 June 2024 9,451 3 View
A need to analyse specific sequences gathered from genbank in order to analyse primers matching/designing. I have used Sequencher (gene codes) in the past and I loved it. Yet prices are now...
24 April 2024 259 3 View
when I insert my primers in primer blast site and push the button "get primers" I just reach different errors and I don't know what the problem is!
22 April 2024 8,001 5 View