Is there a way to align two genomes from the same species but sequenced differently?

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Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020?

Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020? Porque requiero conocer tesis de posgrado nivel maestría...

24 July 2024 5,494 1 View

How much 1X TAE buffer should I use in preparing 1.2% agarose gel?

Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...

12 July 2024 2,058 5 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

Why and how to solve unmatched forward and reverse sequencing result by Sanger sequencing?

I’m currently working on polyglutamine disease by Sanger sequencing to count the CAG repeats. However, since my targeted genes involve CAT or CAA interruption in the CAG repeats, their...

01 July 2024 8,328 5 View

Microchannel fabrication using Su-8 photolithography tear off after soft lithography?

I am using SU-8 2025 to generate a high aspect ratio microchannel. I made two microchannels with the properties of: 1. width = 10 um. height = 25 um. SU - 8 2025. 2. width = 15 um. height = 37.5...

27 May 2024 6,862 2 View

Can Kluyveromyces marxianus ferment maltose?

The yeast K. marxianus is well known for its ability to ferment de disacharide lactose. It is also able to ferment the disaccharide maltose or not? If you can share citations with respect to this...

19 May 2024 7,309 3 View

How to model variable thickness composites in LS-DYNA?

I am trying to model a variable thickness composite part in LS-DYNA and I am unsure on how to implement this. I have used PART_COMPOSITE in the past when testing with a composite part with the...

23 April 2024 9,376 1 View

A y relevant articles related to teaching evaluation system in China?

I am do a research on teaching practice evaluation in China, specifically in Xi'an Shaanxi province

08 April 2024 9,072 1 View

How can I filter a contamined compound?

I have screened a series of synthetic compounds on cancer cells, and some of them were contaminated. These compounds were diluted in 100% DMSO. With a low volume of the compounds (800-1000 µl), I...

25 March 2024 4,740 0 View

Why hapolid cells show no growth after yeast tetra dissection?

Greetings to science community, I am operating Kluyveromyces marxianus yeast and want to generate haploid cells from diploid cells. However after 4 day since dissection, I found no growth of...

09 March 2024 8,360 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Mass spectra averaging algorithm?

I am now developing a python module for ms2 database searching, would like to realize a function that similar to what Xcalibur did, choose multiple mass spectra and get an averaged spectra. But...

22 July 2024 3,975 1 View

How to use NCBI datasets ?

I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...

20 July 2024 1,339 2 View

Enquire about the calculation of percentage of reads mapped to the reference sequence?

Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...

20 July 2024 9,705 0 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View