Im looking to test how a construct can increase the cytotoxicity of t-cells. I was wondering if there is anyone knows of a cell line that is capable of granzyme and IFNy release that I could do a pilot study before I work with PBMCs. Thanks
Dear Elliot, as a rule, CTL lines are unstable. You can establish such line by repeated restimulation with stimulator (allogeneic or transfected) cells at the presence of IL-2. You will be capable to propagate such cell line during several months, but unlikely more. On restimulation you will see IFN gamma release and cytotoxicity. All known to me trials to make immortalised CTL lines were unsuccessful and transformation was associated with the loss of CTL activity. Therefore, obviously, investigators develop such lines to perform experiments during restricted time interval.
I believe KHYG1 cells (sold by DSMZ) are cytotoxic T-cells and have permanently activated granules (Suck et al 2006). I think they have been used as you wish but you might want to have a search on the web for the uses of these cells before buying them . They grow ok but I would avoid TALL104 cells as we have never had much luck growing them.
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