Where can I purchase the restriction enzyme Alf1 to use with 2b-RAD library preperation?

14 August 2017 1 285 Report

The 2b-RAD protocol (Wang 2012) calls for the Alf1 restriction enzyme to digest samples. The BSAXI enzyme is listed as an alternate; but it is not heat inactivated, and I'm unsure how this will impact the protocol.

Thermo-Fisher used to see the product under the code ER#1801, but they no longer carry it (maybe discontinued in 2015?).

Any advice is appreciated.

Elliot Weidow

Thanks Muhammad, they no longer carried ALFI either, but I found an alternative type IIB enzyme (BCGI).

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