I am trying to isolate the intestinal epithelial cells and lamina propria cells from mouse small intestine.
And the thing is... I've tried several protocols from JOVE(Geem, D., Medina-Contreras, O., Kim, W., Huang, C.S., Denning, T.L. Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine. (2012)), and from Methods in Molecular Biology 612 (Stephanie L. Sanos and Andreas Diefenbach. Isolation of NK Cells and NK-Like Cells from the Intestinal Lamina Propria. (2010)). But it didn't work out like other studies had, getting both cell populations with high quality. It's either epithelial cells or Lamina propria cells, have the reasonable result...
Is there any method can obtain both cells with high purity and viability?
Try this one:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266894/
Try this one: Smelt MJ, de Haan BJ, Bron PA, van Swam I, Meijerink M, Wells JM, Faas MM, de Vos P. Probiotics can generate FoxP3 T-cell responses in the small intestine and simultaneously inducing CD4 and CD8 T cell activation in the large intestine. PloS one. 2013 Jul 4;8(7):e68952.
It worked great in my lab
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology