I have attempted various methods for cell lysis in colony PCR, but none have been successful. The methods I tried include boiling the colonies in 20 µL of buffer (1X TE with 0.1% Triton X-100 and 0.2% SDS). In another method, I used 0.2% SDS buffer, boiled the colonies, and tested with 16S primers, but no results were obtained. If anyone has any suggestions or optimizations for this process, I would greatly appreciate your advice. Thank you in advance.