Mohd Asalam

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Questions related from Mohd Asalam

Trying for gene complementation Pseudomonas after creating mutant with vector pK18mobsacB but the plasmid is not getting transformed in my microbe?

Good morning everyone I have created the pseudomonas oryzihabitans mutant via Gibson assembly (homologous recombination) with the suicidal vector pK18mobsacB . And now I am trying for...

17 November 2024 2,892 2 View

Is there a specific colony PCR protocol for Pseudomonas oryzyhabitans? I am facing issues with cell lysis using traditional colony PCR methods?

I have attempted various methods for cell lysis in colony PCR, but none have been successful. The methods I tried include boiling the colonies in 20 µL of buffer (1X TE with 0.1% Triton X-100 and...

10 October 2024 8,678 2 View