When we tried isolating genomic DNA from buccal swabs, high protein contamination was seen. We followed a simple procedure, using SDS to disrupt the cells and precipitation using ice cold ethanol and 5 M Nacl.
Procedures using resins are very efficient; commercially available kits such as
http://www.promega.com/~/media/Files/Resources/Protocols/Technical%20Bulletins/101/DNA%20IQ%20System%20Small%20Sample%20Casework%20Protocol.pdf
or you can check this publication:
http://europepmc.org/abstract/MED/1867860
Thank you very much Dr. Palau for your reply.
but, the general problem we faced was that we need human DNA for our course works and isolation of DNA from blood requires relatively high volume of sample, it's sort of time consuming and as well as an invasive procedure (for blood collection). while i was looking for alternatives i found a few kitchen based protocols on how to isolate genomic DNA from buccal epithelial cells. We tried to modify the protocol using a phenol-chloroform step, but we obtained highly fragmented DNA and relatively higher protein contamination. kit based methods seem to be far efficient but due to their cost, we haven't been able to use one and i was looking for an efficient way to isolate genomic DNA from other tissue samples and buccal swabs; non kit based protocols.
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