I need a robust publication that contains the HPV-16 and HPV-18 specific primers set.
If anyone has the forward and reverse primer of HPV-16 and HPV-18 please write to me.
I reviewed several papers and there were differences in sequence.
Thanks
Hi Maysaa
There is a common primer set for identification of any HPV type as 16 & 18. I've used them and worked properly.
primers; MY09(5-CGTCCMARRGGAWACTGATC-3)
and MY11 (5-CMCAGGGWCATAAYAATGG-3), which
amplify a region of 449–458 nucleotides (depending on
HPV type) of the highly conserved L1 ORF.
It's published paper;
'Complete genotyping of mucosal human papillomavirus using
a restriction fragment length polymorphism analysis
and an original typing algorithm'
Rui Jorge Nobre a,b,∗, Lu´ıs Pereira de Almeida b,c, Teresa C. Martins
If any problem don't hesitate to call me
Good luck
Thank you very much Davod for the detailed answer and for the papers . I really appreciate it
Hi Maysaa. I rather use type specific primers in case of HPV 16 and HPV 18. These sets are old, but are based in very conserved sequences from genes E6 and E7, so they still work. Remember PGMY primers are consensus, so they will amplify every HPV genotype they find in the sample. They work, but you'll have to check with a probe or sequencing if the amplicon belongs to HPV 16 or HPV 18.
I am sending you the references, since I dont have my laptop with the pdf files. Also I am sending you a table with the sequences of the mentioned primers.
Best,
Vanessa
- J Virol Methods. 2000 Aug;88(2):125-34.Comparison of five different polymerase chain reaction methods for detection of human papillomavirus in cervical cell specimens.Husnjak K1, Grce M, Magdić L, Pavelić K.
- Anticancer Res. 2001 Jan-Feb;21(1B):579-84.Evaluation of genital human papillomavirus infections by polymerase chain reaction among Croatian women.Grce M1, Husnjak K, Bozikov J, Magdić L, Zlacki M, Lukac J, Fistonić I, Sikanić-Dugic N, Pavelić K.
Dear Vanessa
Many thanks for you. I really appreciate you detailed answer. but my concern is if I use specific primers for HPV-16 and 18, I afraid to miss another HPV high risk types like 33, 58, 34,62,....etc.
So I think I have to use the primers of L1 (conservative) then make a genotyping or sequencing....
Regards
Maysaa
Is there a publication contain the HPV-6 and 11 primer specific set?
I need a reference publication that contains the HPV-6 and HPV-11 specific primers set.
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