Saliva collection for DNA extraction as micro-RNA expression profile in oral cancer.
If anyone has experience handling saliva samples, please let me know.
Hello Maysaa,
We have done some work with David Wong's group at UCLA and Dr Wong has published a paper in Clinical Chemistry some years ago describing the procedure called Direct Saliva Transcriptome Analysis [DSTA]. This is a highly functional method, but is quite time consuming and tedious, so we have worked on a tool, which is called RNAPro-SAL that is used to collect saliva from subjects in approximately 1-2 minutes using an absorbent pad. The saliva collected is squeezed through a compression tube and then through a secondary filter, which removes cells and larger particles such as DNA and other interferants and is then bifurcated into two Eppendorf or similar tubes, providing two fractions of approximately 0.5 mL of purified saliva. One may be stabilized to look for RNA and the other for proteins or the two samples combined to provide a larger volume sample to look at either RNA or proteins. We have characterized the purified saliva samples and are able to adequately see a number of in house reference genes for miRNA and mRNA in our RNA stabilized sample and a high concentration of purified proteins using calculation of total proteins and an individual protein [IL-8].
This is a very new tool for researchers, so if this sounds interesting let me know.
I would be happy to provide further information on either the DSTA method or the RNAPro-SAL Device.
If this is the case, please respond by e-mail to [email protected] so I can send the relevant files.
Kind regards
Paul
Paul D Slowey PhD
CEO, Oasis Diagnostics Corporation
E-mail: [email protected]
Thank You Dr. Slowey
Yes I am very interesting with this methods. I wil contact you via e-mai.l
I have contacted to Dr. Wong in UCLA for my project in an attempt for trainning in his lab but he did not reposond to my e-mail.
However, I thaink it would be very helpful to me to try your tool
since here in Iraq there is no previous expirence in RNA extraction from saliva as far as I know.
Thanks again
Hello Maysaa, I will be visiting Dr Wong's lab at UCLA this coming Friday so if you would like me to speak to him about training in his laboratory please let me know.
If you have a resume you would like me to pass on to him, please send this via e-mail. I did just respond to your earlier e-mail with information on the DSTA Method and also the RNAPro-SAL device.
Best regards
Paul
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