Reshma Reddy

4 Questions 3 Answers 0 Followers

Questions related from Reshma Reddy

Usage of some fold higher value of IC50 (For eg: 4fold or 10fold)for development chemoresistant cell line. Explain?

For development of chemoresistant cell line some papers mention about usage of 10 fold or 4 fold higher value of IC50 for short duration of drug exposure. How to calculate the fold value to be...

01 January 2019 6,889 0 View

Has any one standardized HPV16 E7 antibody for Western blot?

Currently I am working on standardization of HPV 16 E7 antibody (1:1000) but I am not able to get band at desired position. Please provide suggestions for the same. Thank you

12 December 2018 5,703 0 View

I am working with cervical cancer cell lines. I want to determine optimum cell density for drug exposure?

I have performed MTT assay with cell densities ranging from 1000-9000 cell and added MTT after 24h of incubation. The resulting absorbance is less than 0.75. Hence I selected higher cell densities...

08 August 2018 1,227 0 View

How to determine number of cells to be seeded to perform MTT assay?

I want to perform MTT assay and for that cell density plays a very crucial role. How to determine cell number to be seeded for a particular cell line.

05 May 2018 10,017 3 View