Is there a protocol to dialyses the lipidated AcrA protein using PBS? or with low concentration of Triton-X?

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How to test a hypothesis with Confirmatory factor analysis using bootstrapping technique?

I am trying to perform CFA uisng AMOS SPSS for a dataset with 34,000 observations. All the features (dataset columns) in the dataset have non normal distribution. The hypothesis that I am trying...

28 July 2020 8,923 5 View

Does any one know the binding residues of human ACE2 which interact with the spike protein of Covid-19 ?

I want to find the residues of human ACE 2 which interact with the spike protein of Covid-19? or can anyone tell me a method to find the interaction residue for this PDB file? PBD ID: 6lzg thank...

10 April 2020 3,071 5 View

What are the methods that can be used study adsorption by using Gaussian.?

Dear sir/ madam I am studying about filtration of pesticides. how can I study the adsorption pesticides to the filtering medium using DFT in Gaussian 09. I have already tired using homo lumu...

06 June 2019 2,572 2 View

How to make a PDB file with the receptor and ligand from docking results from autodock.?

I have docked a ligand with a perticular receptor and obtained the most stable 10 binding place. i need to visualize the binding site using pymol and i only can input pdb files to pymol. how can i...

29 May 2019 1,932 6 View

Where can i find the structure file of polyacrylamide to be using in Gaussian to study it's properties ?

I am hopping to use the structure file to study of polyacrylamide to study its homo lumo levels using DFT in gaussian 09w and to use the structure in molecular mechanics to study the adsorption...

26 May 2019 4,767 4 View

Does anyone know an efficient method to identify/sequence transposons insertion sites?

I am using Ez-Tn5 transposns system to create random mutations in E.Coli. After all the screening procedures I have narrowed down the samples I want to proceed for sequencing. However, I have...

30 March 2019 4,897 4 View

What are the indicator species to have idea on climate change?

I think some of the lower plant species (fens, moss, etc...) can use as indicator species, but more infomations are not available for prove that idea.

23 December 2011 3,704 16 View

What is the most suitable basic set (DFT) for heavy metals such as Hg, Pb, Cd, Cr, Ni and etc ?

I am looking for a reliable basic set for heavy metals up to the 6th row. I am looking forward to carrying out these calculations using gaussian 09. What is the most suitable basic set for my...

01 January 1970 5,672 13 View

What is the best CPU for running Gromacs and Gaussian ?

Hello everyone. I am planning to build a workstation capable of running both gaussian and gromacs. I have planing to use RTX 3080 as the GPU and 64GB memory. However, I have not able to select a...

01 January 1970 7,354 2 View

The NAMD2.exe fails to startup. How can I overcome this problem ?

I have tried all the suggestions on the internet but failed to correct the above error. The application was unable to start correctly (0xc000007b) can someone tell me how to overcome this...

01 January 1970 8,820 5 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View