Does PFA transcardial perfusion artefactually stretch the rodent heart?

Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...

28 October 2020 8,059 2 View

Is this typical lymphocyte, the flower cells?

21 September 2020 4,107 3 View

Which is better in determining the effectiveness of artificial licks for wildlife detection in forested areas?

Hello, I'm planning for my future research study on artificial salt lick. The study site that I've selected for this study has been confirmed with no presence of natural salt lick, thus I plan to...

02 August 2020 5,609 1 View

What statistical tests can I perform on glycomics data collected from disease cohort across 3 timepoints?

I have a dataset comprising clinical characteristics and glycomics profiles of disease samples (n=100+) and matched healthy controls. So far, I have been able to do classification modeling to...

20 July 2020 2,153 3 View

0.5M EDTA buffer preparation: Why does it turn into milky white foam-like mixture?

I am making 200ml of 0.5M EDTA, pH8. I mixed 29.22g EDTA (MW: 292.24g/mol) with 100ml distilled water first (planning to add up to 200ml after measuring pH). I was adding NaOH pellet into the...

03 June 2020 4,581 3 View

How to grow SnO2 nanorods on FTO substrate using hydrothermal method?

i try to immerse SnO2 thin film cotaed-FTO substrate on aqueous solutions (DI water, ethanol and small amount of HCl ), and the experiment is carried out inside lab oven with the temperature of 90...

12 January 2020 4,419 4 View

Which is the best instrument to measure social competence in 12-year-old children?

i am having difficulties in choosing the one and best suitable instrument of social competence for the 12-year-old children. i am planning to combine different questionnaires to measure it, but...

11 December 2019 1,591 3 View

Apart from western blot, what are the other tests that can be used to determine expression of autophagy-related proteins ?

I would like to know if there are other options that requires less cost.

09 July 2019 6,974 2 View

Flow separation : Turbulence or Laminar?

Hi researchers, I am performing CFD analysis inside an electronics enclosure. The Reynolds number is below 1000 but I have some blocks and heat sinks inside that creates flow separation and wake...

20 May 2019 1,357 19 View

Social competence scale for grandparents-raising grandchildren?

Hi, I am Hoon. I am currently working on my master thesis on grandparenting and its influences on child's social competence. i failed to find suitable child social competence scale for 12-year-old...

28 March 2019 8,832 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

What is the right way to conjugate two acids?

How to conjugate two acids with amine linker?

18 February 2021 4,083 2 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View