I would like to perform phagocytosis with insect cells using zymosans that are conjugated with a suitable coloured dye (non fluorescent). However I cannot seem to find a suitable protocol to do that. Please help, thank you!
Hi everyone! After thinking about it on my own, I'm posting a question here. Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target...
30 June 2024 1,569 1 View
How to purify boron esters? Hello. I'm synthesizing borates in my lab. My questions are as follows: 1. How to purify boronate esters like B(OR)3 compounds, and it is a UV undetected...
23 May 2024 7,072 2 View
We are currently doing community work where we have to measure the height and weight of children 6-10 years old. The community work is done across different communities. Thus, since the...
03 April 2024 7,311 0 View
Hi All, I would like to seek for your expert input for below queries on RNA QC for RNASeq: Can we proceed for RNASeq run on RNA less than 10ng, with DV200 in between 68 - 80%?Details as below:...
18 March 2024 5,936 2 View
I am currently conducting research on purifying Myoviridae phage particles using sucrose density gradient ultracentrifugation. However, I have encountered a challenge where no visible opalescent...
12 March 2024 9,387 3 View
hi I just start building a nonmem pharmacokinetics model. i got a problem with my estimate result .. is there any reason for %RSE=0?? if anyone know about this issue , please explain this...
04 March 2024 6,077 0 View
I'm following a protocol to bind fluorescent ligand to receptors expressed on cells in a microplate. The protocol includes fixing cells with 4% PFA for 20 mins. This prevents cell loss during...
09 January 2024 7,517 7 View
Looking for somebody who had similar experience when you use DOI issued by ResearchGate. I published one paper in a conference in 2007. The conference seems not to publish their proceeding, and I...
25 December 2023 5,537 1 View
Required a research paper with raw data to conduct a comparison of recent research and future direction
05 December 2023 2,528 1 View
Could I obtain a compact film through the atomic layer deposition method? If yes, why as-deposited SnO2 film with a thickness of 17 nm fail to protect the underneath layer film of my device? Could...
05 November 2023 8,153 0 View
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...
29 July 2024 1,981 1 View
Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color
28 July 2024 286 0 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
I used Aqua regia to clean Au nanoparticles in the used glassware. But for some reasons, it is not heating up upon mixing. The color did change from no color to light orange. The Au NPs on the...
25 July 2024 4,390 0 View
For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?
23 July 2024 8,008 1 View