I would like to follow the growth of bacteria with life-cell microscopy. I reckon I will need a 100x objective, but since these are immersion lenses, how can I follow several colonies in the same run?
Is there a good protocol for this kind of experiments?
On Pubmed there are thousands of papers, but mostly they use fluorescent dyes. I just need to see a bacterium dividing into a colony.
Thanks
You can set up the bright field imaging at 1 minute per frame for example. You can also use the Bio-station microscopy for tracing cell division for prolonged periods.
Thank you, but I understood the point of this live imaging was to use the z-option of the microscope to check several colonies in the same session. But how can I move an immersion lens without disrupting the cells?
I found this protocol
Article Live Cell Imaging of Bacillus subtilis and Streptococcus pne...
essentially, one uses a slide hence it is possible to slide the lens on different areas of it without detaching it. Thus, one can use a 100x immersion and very few bubbles are expected.
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