I'm using a U bottom plate for the protocol. I want to check if my material can activate these cells by measuring the IL-2 release. I'm not sure about the required number of cells/well, incubation time etc.
Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss.
IL-2 production should be measured 6 to 12 h later (whatever works best for you).
0.5 to 2 millions cells per ml works. We have used the Jurkat E6.1 variant in our assays.
I hope this works for you.
Good luck.
Hi
Anti-cd3/cd28 Ab and PHA activate Jurkat T cells and induced IL-2 production. Also, you can refer to the following article: 10.1172/JCI112464
I activate the CD8-T cell and perform ELISA for IL-2, and PFP but the value of negative ctrl and ctrl are the same, which means the cells are not activated. what is the specific procedure to know about the activation confirmation?
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line