18 March 2016 3 2K Report

using lysosome emzyme

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Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View

What is the most accurate way to determine the energy gap of semiconductor materials? Is it better to use UV-Vis or PL measurements?

When preparing hierarchical zinc oxide ananostructure, the energy gap measured by PL was equal to (3.26 eV) While the energy gap value was determined by UV-Vis measurements (3.5 to 3.7 eV)

26 June 2024 7,505 6 View

What is the most accurate artificial urine recipe used in vitro for microbiological purposes?

I just found many protocols but not specific for bacterial growth

30 May 2024 6,305 2 View

How can I explain the acid base titration test for a PH responsive nanogel?

I have three nanogels.. The difference between them is the cationic monomer ratio... However, the test is giving me similar curve of the three formulations

27 May 2024 2,064 0 View

Question about XRD?

Hello, I was elaborating thin films from FeCl3 using a dip coater. These films were deposited on a glass sheet for 6 layers and then annealed at 500°C. However, XRD analysis did not yield any...

31 March 2024 9,413 3 View

Can I use two IRIS in the same plasmid expression cassette ?

I am trying to express m6A protein and a fluorescence one RFP in the piggyback vector. I was thinking first of using the P2A to separate between the m6A and the RFP protein but then I was afraid...

12 March 2024 9,713 0 View

How can I encapsulate cisplatin into a ph responsive polymeric nanogel?

I have synthesised a 100 nm sized cationic nanogel and I want to use it as a carrier for cisplatin and paclitaxel

03 March 2024 3,426 0 View

What tools needed to conduct areserch study about "burden of mothers in caring for child with colstomy" besides zarit burden interview scale ?

i'm preparing my protocol for master degree and it's my first time ,i need some help ,my reserch titel is "burden of mother in caring for child with colstomy"

02 March 2024 5,656 2 View

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Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

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I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

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Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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