22 October 2019 2 2K Report

Hi, I'm planing on integrating into the genome one fragment containing three genes (one of which is a resistance gene) in this manner:

Gene1 -> Resistance Gene -> Gene2 ->

The arrows indicate the reading frame direction.

Every gene has it's own promoter and terminator.

I was wondering if someone has experience with such cassettes, is there a problem with having the genes close and in the same direction? I was thinking it may cause a promoter to work in the opposite direction and therefore create an antisense for one of the genes and silence it.

Thanks

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