Is it accepted/required/desirable/appropriate that the supervisor recommends that the student review the supervisor's previous work and publications?

Assuming that doctoral candidates are usually coupled with supervisors based on areas of academic interest which they have in common, to what extent is it customary for the supervisor to discuss...

21 November 2019 5,399 7 View

Is there a promblem with tandem insertion of 3 different gene cassetes in S. cerevisiae?

Hi, I'm planing on integrating into the genome one fragment containing three genes (one of which is a resistance gene) in this manner: Gene1 -> Resistance Gene -> Gene2 -> The arrows...

21 October 2019 1,488 2 View

Cannabidiol powder solubility and stability?

Hello, I'm interested in knowing if cannabidiol is stable for long term storage in methanol or DMSO in recommended solubility mix for biological studies and in which conditions. Thanks.

21 October 2019 2,445 1 View

Which resources could help residents develop a successful grassroots participatory process ?

In my article "When knowledge is power: grassroots participatory initiative as a process of resource development" I claim that residents are initiating informal grassroots participatory...

25 December 2017 1,657 12 View

Problem mixing the aqueous phase with isopropanol - RNAlater samples

Hallo everybody, I was extracting RNA (with TRIzol protocol) from bees in RNAlater. At the precipitation step we found that the aqueous phase was not mixing with the isopropanol - as if they were...

21 June 2017 6,379 1 View

Does anyone have experience working with GP120 and SF9 cell transfection?

I am currently trying to perform a transfection of GP120 baculovirus into SF9 cells. However, my western blot keeps showing large numbers of nonspecific bands that show up even in the negative...

02 December 2015 5,332 1 View

Can I select for IPTG-induced fluorescent e. coli colonies on LB-Agar?

I am doing recombineering and would like to swap out GFP for mCherry in my vector. I can proceed with the GalK selection scheme but I tried it once and didn't work so I am trying to think of...

05 October 2014 1,713 3 View

No title

01 January 1970 7,982 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

What/How to focus on with regard to research publication?

I'm an academician for past 10 years and love to continue with the same, a question pops-up at in mind from time to time, what to focus more on? Conference papers, Journal paper, book chapters,...

09 February 2021 1,009 5 View

The litter effect and what can I do to avoid it?

My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...

25 January 2021 6,965 3 View

Is there a kit to detect COVID-19 in freshly isolated human primary cells?

Our lab harvests HUVEC (human umbilical primary endothelial cells) and endothelial cells can support COVID19 growth. The mothers will be screened for Covid, but we all know that there are false...

14 January 2021 8,530 2 View

Most of the plastic is shipped to Asia?

Dear Sir I came across your paper. First of all, congratulations on your amazing research article. Loved it! Also, I am very interested to know, why is the waste that is collected in the...

14 December 2020 6,342 4 View

How to call this type of study please?

Hi there! :) I'm working on creating a follow up study and I need help determining what type of study I'm going to be doing whether its 3x2 or 3x3 please? Details: - I have 2 groups of children...

26 November 2020 4,380 2 View

Does anyone know of a good PAX7 antibody for IF - not from the DSHB?

Hi, Although I would dearly love to obtain a PAX7 Ab from DSHB, it is not on our university ordering system (and it takes too long to add it). So I wondered if anyone had good experience of a PAX7...

03 November 2020 7,003 2 View

How plant taxonomy is the mother of botany?

Research articles

11 October 2020 380 19 View

Is there a solvent for blending PHB and Acid Ascorbic (via solution casting)?

Dear Researchers I want to add Ascorbic Acid (AA) to PHB in order to improve its biodegradability. AA is soluble in water but insoluble in those that are compatible with PHB (THF, Chloroform,...

07 October 2020 1,136 4 View