Is it accepted/required/desirable/appropriate that the supervisor recommends that the student review the supervisor's previous work and publications?

Assuming that doctoral candidates are usually coupled with supervisors based on areas of academic interest which they have in common, to what extent is it customary for the supervisor to discuss...

21 November 2019 5,399 7 View

Is there a promblem with tandem insertion of 3 different gene cassetes in S. cerevisiae?

Hi, I'm planing on integrating into the genome one fragment containing three genes (one of which is a resistance gene) in this manner: Gene1 -> Resistance Gene -> Gene2 -> The arrows...

21 October 2019 1,488 2 View

Which resources could help residents develop a successful grassroots participatory process ?

In my article "When knowledge is power: grassroots participatory initiative as a process of resource development" I claim that residents are initiating informal grassroots participatory...

25 December 2017 1,657 12 View

Problem mixing the aqueous phase with isopropanol - RNAlater samples

Hallo everybody, I was extracting RNA (with TRIzol protocol) from bees in RNAlater. At the precipitation step we found that the aqueous phase was not mixing with the isopropanol - as if they were...

21 June 2017 6,379 1 View

Does anyone have experience working with GP120 and SF9 cell transfection?

I am currently trying to perform a transfection of GP120 baculovirus into SF9 cells. However, my western blot keeps showing large numbers of nonspecific bands that show up even in the negative...

02 December 2015 5,332 1 View

Can I select for IPTG-induced fluorescent e. coli colonies on LB-Agar?

I am doing recombineering and would like to swap out GFP for mCherry in my vector. I can proceed with the GalK selection scheme but I tried it once and didn't work so I am trying to think of...

05 October 2014 1,713 3 View

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01 January 1970 7,982 1 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View