Hi there,
for a continuous enzymatic reaction (membrane reactor with immobilized enzymes), I am looking for a buffering substance that is not or only slowly degraded by thermic or other processes. pKs should be around neutral.
Phosphate would be the best choice, but I cannot use it due to its chelating properties for divalent cations which are a vital component of my solution.
Tris would be the next option, but it is quickly degraded over time as I recall correctly...
I do not want to let it all fail because the buffering substance gives up even before the enzyme dies...
Any ideas?
best regards,
peter