Can I use G418 for selection of HEK 293 cell lines that already have a plasmid with kan and Hygro integrated?

I am doing a kill curve in HEK 293 cells with G418(0-1000ug/mL) and they don't die. They were previously transfected and selected with hygromycin and are integrated with a plasmid has also...

05 December 2020 3,232 3 View

Adaptability of frugal health care systems into developed markets - can someone share ideas on methodology for dissertation?

Hello! Hope everyone is doing alright during these turbulent and uncertain times. I am working on my masters dissertation (MSc International Management) and would like to answer the research...

05 August 2020 6,155 4 View

Do Transformational Leadership can be conducted qualitatively?

Most of the Transformational leadership study uses a quantitative method by doing a survey with an instrument that can be found. Is it possible to conduct it in a qualitative method, specifically...

16 July 2020 1,815 3 View

What is the best production evaluation method in an architectural design company?

I would like to know what is the most effective method to manage processes in an architecture company

29 April 2020 2,932 1 View

Dissertation topic for MSc International Management?

Hello, I am doing my masters in international management and looking for a research question related to start-up/ authenticity/ crowdfunding/ entrepreneurship & innovation to do my dissertation...

21 April 2020 5,222 23 View

What are the evidences of the AI's impact on the company's scalability?

How could we differentiate a usual scalability (organic or without AI technologies application) from an AI-impacted scalability of the enterprise?

27 August 2019 3,081 4 View

Plasmatic proteins solvent?

Can anyone suggest me a solvent to solubilize plasmatic proteins? I tried with formic acid, but was parcially disolved. Thanks

03 July 2019 10,030 2 View

How can I induce sickling consistently with sodium metabisulfite?

Hi all I'm trying to perform the procedure of sickling-induction in-vitro of peripheral red blood cells from sickle cell Townes mice, and I'm using 2% sodium metabisulfite. Unfortunately the...

20 January 2019 1,214 1 View

Hi, Is it normal when run PROCESS macro in SPSS for moderator (model 1) to get different results from when I run the linear regression in SPSS?

Could you please help me by giving me some advice on this? Thanks.

02 August 2018 2,012 2 View

Can I use at least 2% of fetal bovine serum in a transfection with Hiperfect?

I'm trying to transfect N1E-115 cells and the HiPerfect protocol advices not to add fetal bovine serum during the transfection phase. Unfortunately, my cells are dying without the FBS. Do you know...

23 October 2017 4,147 4 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View