Greetings,
I'm having difficulty understanding my results from doing a ponceau S stain on a nitrocellulose membrane after western blot. I loaded 10ug in lanes 2,4, and 6. 15ug in lanes 3,5,and 7. The 2&3 lanes are goat testes and 4&5 is bovine liver and 6&7 is bovine adipose tissue. I'm more familiar will RNA and EtBr gels and looking for degredation, etc.
Valeria De Arcangelis
Hi, maybe you have resolve your problem but I think you have to improve some gel condition, and liver samples preparation.
Did you make coomassie on gel, out of curiosity?
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