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Questions related from Sebastián Montealegre
I would like to nucleofect human BJ1 hTERT immortalised fibroblasts with a RNP complex made of Cas9 and a single crRNA+Tracer RNA, using the Amaxa 4D cell nucleofector device. Is there a standard...
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I have been trying to clone mCherry from another vector into pLVX. mCherry is flanked by a NotI site at the 5' end and a BamHI site at the 3' end. The two sites are present into pLVX contigous one...
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I want to use HEK 293T cells for the production of lentiviral particles. I was told that after thawing the cells from the liquid nitrogen, I should split them 4 times in the presence of 1 mg/ml...
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