Sebastián Montealegre

2 Questions 3 Answers 0 Followers

Questions related from Sebastián Montealegre

Is there a good protocol to nucleofect Cas9+sgRNA RNPs into BJ1 hTERT cells?

I would like to nucleofect human BJ1 hTERT immortalised fibroblasts with a RNP complex made of Cas9 and a single crRNA+Tracer RNA, using the Amaxa 4D cell nucleofector device. Is there a standard...

02 March 2022 2,561 0 View

Is it necessary to pre-treat HEK 293T cells with Neomycin before using them as packaging cells?

I want to use HEK 293T cells for the production of lentiviral particles. I was told that after thawing the cells from the liquid nitrogen, I should split them 4 times in the presence of 1 mg/ml...

12 August 2015 8,970 3 View