Is there a dilution factor for trypan blue exclusion assay, when we use 90 micromolar cell sample and 10 micromolar trypan blue?

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Steingrimur Stefansson

Hi Khatereh, please take a look at this protocol:

http://www.lifetechnologies.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/trypan-blue-exclusion.html

Jordan R. Yaron

From a 0.4% solution we use 1:1 with cell medium (10 uL of each and 10 uL of that solution) onto a Countess automated counter slide. For manual counting 1:10 is recommended I believe.