I have been having difficulty differentiating C2C12 cells into myotubes. I use DMEM media with high glucose + glutamine + sodium pyruvate supplemented with 2% horse serum and 1% penicillin/streptomycin. However, I have noticed most published work on C2C12 cell line use DMEM high glucose + glutamine, no sodium pyruvate. Could the presence of sodium pyruvate in my media be affecting my differentiation?
Is sodium pyruvate not suitable for C2C12 cell lines?
Hi Magdaleena Mbadhi . Carbohydrate metabolism can affect c2c12 differentiation.
Article Carbon metabolism-mediated myogenic differentiation
Hi Magdaleena Mbadhi! Does it work after you change the medium without sodium pyruvate? Thanks!
Hi Liang Yusheng . It took a while to get the media without pyruvate as it was not available in my region, I had to wait for it. I am currently busy starting with differentiation of the cells, I will update if I got any difference once I get my data
Liang Yusheng Hi. To follow up on your question. I obtained my data for C2C12 differentiation on days 2, 4 and 6. I don't believe the media without sodium pyruvate made that much more significant difference than when I was culturing with sodium pyruvate containing media. I see a slight increase in my gene of interest expression. However, it is not very significant and multiple factors could have contributed to it. Hence, I can't say with full confidence that the media without sodium pyruvate is indeed better for differentiation with these cells. I hope this was helpful
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