Magdaleena Mbadhi

3 Questions 7 Answers 0 Followers

Questions related from Magdaleena Mbadhi

How best can I induce an inflammatory response using LPS in immortalized human myometrial cell lines (hTERT)?

I've been trying to induce an inflammatory response using LPS (Sigma O127:B8 and O55:B5) using the immortalized human myometrial cells (TERT-HM) in our lab but have failed to get a significant IL6...

13 July 2023 1,673 3 View

Is there a difference between Sodium pyruvate vs no sodium pyruvate contanining-DMEM for C2C12 culture?

I have been having difficulty differentiating C2C12 cells into myotubes. I use DMEM media with high glucose + glutamine + sodium pyruvate supplemented with 2% horse serum and 1%...

05 July 2021 7,083 5 View

Why do I have a low protein concentration?

I recently extracted my protein from day0, day2, day4 and day6 cell culture and performed a BCA. I intend to carry out a western blot, however, the protein concentration obtained from the BCA of...

22 October 2020 5,561 5 View