How to prepare 5% Glutraldehyde solution from 25% Glutradehyde in water?

Hello all, I need to prepare 5% Glutraldehyde in 0.2 M phosphate buffer. I have 25% Glutraldehyde solution in water in liquid form. Kindly suggest me the step by step protocol to achieve this.

25 September 2019 4,901 2 View

How to purify low polar compounds in PE fractions?

I am isolating compounds from plant extract from a Petroleum ether fractions. There is some compounds in the elution of 5% Ethyl acetate:95% Petroleum ether fractions as clumped and it is hard to...

25 July 2019 7,873 3 View

Why is the torque recuction 50% at bolt joints for metal foam sandwich?

I have a part which is integrated with foam in the back is bolted to another Metal part. i find the torque is not applied completely while using pneumatic or electric gun. No issues observed while...

20 December 2015 9,656 2 View

Can someone help me with 2D Modeling of composite in abaqus ?

12 March 2015 5,782 4 View

Can anyone give some general properties for 2D modelling of breakage of composites in abaqus?

2d modeling of composite in abaqus the tool is not broke the composite material why? which property I want to be given to the tool property to form a chip....now stress only forming....on striking...

10 March 2015 8,927 1 View

Why do I get an error when 2d modelling of machining composite in ABAQUS?

06 March 2015 9,827 5 View

Which polymer is suitable for improving the linear thermal expansion coefficient?

what kind of polymer added to improve the linear thermal expansion co-efficent property?

24 February 2015 5,312 2 View

How its write fortran code in abaqus on machining of composite? can any one suggest the which books to refer?

I am 3D modeling machining of composite in Abaqus. But it is not working I don't know anything about how I can run.

06 February 2015 5,564 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View