Is there a Competetive peptide for GFP?

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Jordan R. Yaron

Competitive peptide release doesn't work for GFP-trap because the affinity of the nanobody is too high. You can elute in glycin/pH2.5 followed by immediate neutralization, or you can elute in SDS sample buffer.

Better would be to introduce an enzyme cleavage site after the GFP.

They released an article specifically discussing this issue:

http://resources.chromotek.com/blog/how-to-elute-gfp-fusion-protein-from-the-gfp-trap

Jaya Aseervatham

Thank you