I am working on doing a transformation with the intent to use that DNA for a transfection. I am finding, however, that the process for doing that is relatively long. Is there a company that would sequence a double stranded DNA oligonucleotide for me that is ~2000 bp long, so I can skip the steps prior to transfection and just purify the DNA with a kit instead?
Hey Chris,
The only company I know of that can do a gene block that large is genscript. Here's a link: https://www.genscript.com/genparts-dna-fragments.html?src=google&gclid=Cj0KCQjwrfvsBRD7ARIsAKuDvMOdGaHz9tCLy6okSvgvhpbwtZES7kmT6JpQ-iDd6SEt93m8Lw6aWgQaAgTsEALw_wcB
Best,
Max
Hey Chris,
You can talk to the IDT, they might help you with this assignment.
https://eu.idtdna.com/pages/products/genes-and-gene-fragments/gblocks-gene-fragments
best wishes,
SD
Hi Chris.
We have ordered dozens of genes from Genscript; some of these genes were as long as 2500-2700bp. They did it perfectly.
Stan
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