Chris Yates

3 Questions 2 Answers 0 Followers

Questions related from Chris Yates

Is there a company that will synthesize DNA sequences that are ~2000 bp long?

I am working on doing a transformation with the intent to use that DNA for a transfection. I am finding, however, that the process for doing that is relatively long. Is there a company that would...

10 October 2019 6,914 3 View

Why do we transform clones into bacteria before doing transfection into mammalian cells, if we will be putting the same DNA into the mammalians cells?

I imagine it might have to do with verifying that the proteins that we want to have in the clonal plasmid are actually there, and that our other procedures would work afterwards, like...

09 September 2019 4,970 4 View

Why should I use a transparent cylinder for the Forced Swim Test? Would an opaque cylinder work if we recorded the rat swimming from above?

We are planning on doing behavioral testing before and after inducing depression. One behavioral test we want to do is the forced swim test. We are wanting to use 20 cm (D) x 50 cm (H) cylinders...

02 February 2019 4,780 1 View