Hello, better way first isolate Alternaria sp. than use easy cheap and reliable benzylchloride method for DNA extraction.

Method is attached.

Good luck.

@Mustafa, the QIAGEN DNA isolation kit (DNeasy Plant Mini Kit) would work just fine. I will recommend it.

Best wishes,

Eky

(PS: I do not work for the company, I only used the kit).

You can use Qiagen DNeasy Blood and Tissue DNA Kit or Qiangen DNeasy Plant Mini Kit.

@ Karen A. Darbinyan

Do you mean that i can separate the two DNAs through this procedure?

Ekemini Obok Yashwant Chavan I have used the miniprep from Qiagen a lot, but it extracts the total DNA (i.e. both fungal and plant DNA at the same time).

It is not able to separate only plant DNA.

Thanks

Hello, by benzylchloride method you can extract both plant and fungi DNA by same procedure and same reaction mixture and you will receive both DNAs mixture. If you want to isolate only fungi DNA the better way is to isolate fungi culture from your material as pure culture and then perform DNA isolation.

Karen A. Darbinyan Thanks.

I have done separate isolation, but a friend mentions that there is a possibility of isolation only plant DNA; I do not think it is possible; that is why I am asking.

Thanks

I think that whenever you do the extraction of infected plant material you will get DNA from the plant and the fungus. If you want only the DNA of the fungus try to make isolation. If your goal is to detect the fungus in the infected material, check if there are specific primers for this fungus and you can use the DNA of the infected plant material. In this case and if the specificity of the primers has been tested against the DNA of the plant and does not react, you will only get a band if the fungus is present.

Maela Leon Thank you so much for the response.

Actually, what you mentioned is the method I am following, but I wanted to make sure if there is more specific kits that targets one DNA of the two.

Thanks again,

As far as I know, I think it's not possible. If you want only the DNA of the fungus, try to isolate the fungus in culture medium and then do the extraction of the culture

Maela Leon Thank you so much

Nice contribution dear Maela Leon