I have tried using APES already but there is some problem in achieving the result. Is there any other method?
how do I know that APES got coated properly on the slide?
Hi, Venkatesh,
I have the answer for you if your nanoparticle cores are coated with surfactant or any organic molecules. This technique is electron beam irradiation. After electron beam irradiation, surfactant outside nanoparticle cores will become amorphous carbon, which hold nanoparticles in their position even under thermal treatment and low dose of oxygen plasma. After e-beam treatment, nanoparticle cores cannot be washed away by any solvent. You can find a lot of details in my following paper:
http://pubs.acs.org/doi/abs/10.1021/nl3032372
if you want to organize your paper in beautiful arrays, here is my another paper:
http://pubs.acs.org/doi/abs/10.1021/nn2037048
you can download them from my research gate.
good luck
If do just dip-coaing or spin coating or whatever, the silver/gold nano-particles could get washed easily. You could do two things, one is to use some epoxy/surfactant (like Triton X-100) with the NPs and do spin coat on glass slides. It may affect the property of your particles. Then you could do heat treatment ~150 degree C to remove some surfactant. Another way would be to use some sacrificial polymer coatings/layers on which you can do your Nps deposition. Also, surface functionalization of the glass slide could give some advantage to your problem.
I'm not a specialist of this chemistry thing, but if I remember correctly there is something about it there : http://people.duke.edu/~bjw24/Publication57.pdf Though it may depend on how solid you want the bound to be, obviously.
My 2 cents.
Hi, Venkatesh,
I have the answer for you if your nanoparticle cores are coated with surfactant or any organic molecules. This technique is electron beam irradiation. After electron beam irradiation, surfactant outside nanoparticle cores will become amorphous carbon, which hold nanoparticles in their position even under thermal treatment and low dose of oxygen plasma. After e-beam treatment, nanoparticle cores cannot be washed away by any solvent. You can find a lot of details in my following paper:
http://pubs.acs.org/doi/abs/10.1021/nl3032372
if you want to organize your paper in beautiful arrays, here is my another paper:
http://pubs.acs.org/doi/abs/10.1021/nn2037048
you can download them from my research gate.
good luck
A common approach is to use nanocomposites by embeeding the NPs in a host matrix (i. e. polymers or metal oxides). By spincoating and other deposition techniques (Dr. Blade, dip coating, etc...) you can generate thin films and even patterns (inkjet printing or lithography). Take a look to my research gate for further info.
If I remember correctly, gold sticks very well to silanized glass. There are quite a few protocols available for that in literature. Silanization (if e.g. you have a monolayer) is no easy to detect, especially since glass slides tend to have own roughness. I would suggest AFM phase imaging (phase is very sensitive to visco-elastic properties and you can typically see the difference between glass and silane). There are also protocols for thiol coating for gold adhesion.
I assume that APES = Aminopropyltriethoxysilane (?). If so, it can be tricky to get a good, stable coating on glass of an aminopropyl silane. The amine functional group also may not be the ideal choice to interact with the nanoparticles. However, for noble metals, a thiol-containing silane is likely to be a better choice. Look for procedures in the literature based on a silane such as 3-mercaptopropyltriethoxysilane. You will need to be careful to avoid moisture during the silane deposition process.
im recently very new to experimenting with nanoparticles and i would like to coat gold nanoparticle onto glass slide as well. I thought APS(amino silane) coated slides with positive charge would adhere to citrate stabilised GNPs. Will it adhere strongly and other biomolecules such as antibodies can be conjugated with the GNP on the slide surface after wards? Thank u
If this discussion is active still, could someone let me know how we may confirm that the monolayer of APTES on the glass slide is uniform. In the papers below, (1) Nath, Nidhi, and Ashutosh Chilkoti. "A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface." Analytical chemistry 74.3 (2002): 504-509. and (2) Nath, Nidhi, and Ashutosh Chilkoti. "Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size." Analytical Chemistry 76.18 (2004): 5370-5378. it has been mentioned that the glass slides must be sonicated 5 times in ethanol. However, every time, I see a white-colored precipitate on the glass slide after the 3 hr, 120 deg C drying step. While following this step, I see gold nanoparticle aggregates on the surface, even if I sonicate the colloidal sample prior to deposition. In my understanding, non-uniform APTES coating is one of the reasons for gold nanoparticle aggregation. It must be noted that I used microscope glass slide instead of cover glass slide. I would like to know why "cover glass slide" is preferred to "microscope glass slide" for chemisorption of gold nanoparticles?
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