What are the parameters for coating SiO2 thin film using a Thermal Evaporator?

I have seen many works reporting the use of thermal evaporation technique but they seldom give details about the source, boat and other detailed parameters. Can anyone guide me with these details?

04 May 2017 8,294 4 View

How do you calculate the electron density of a nanomaterial?

08 September 2014 691 7 View

How to determine fluorescent decay rates (radiative and non-radiative) of a dye?

I have a nanosecond pulsed laser. How can I find out radiative and non-radiative decay rates of a dye molecule using this nanosecond pulsed laser? Can you suggest some simple experimental methods...

07 August 2014 3,165 14 View

How can I prepare carbon dots with absorption maxima around 405nm?

I have read about synthesis of carbon dots by various means like thermal and microwave mediated. Does anyone know a reliable method to synthesize carbon dots which have absorption maxima around or...

02 March 2014 5,381 1 View

Which polymer is both permeable to lower alcohols and is also insoluble in them?

I'm finding it difficult to find a polymer that is permeable and insoluble in lower alcohols like methanol, ethanol and isopropanol.

08 September 2013 9,801 4 View

Can anyone suggest a good CVD or PVD equipment?

The applications may include metal thin film deposition, semicondicting layer deposition and carbonaceous thin film deposition. Which one is preferable with respect to cost, ease of working etc?...

08 September 2013 464 8 View

Is there any other way to deposit Nanometer silver thin film other than using CVD or PVD instruments?

Can we deposit silver or gold thin film of nanometer thickness using techniques other than vapor deposition techniques?

08 September 2013 7,947 6 View

Is there any software to convert an image into a spectrum or to get XYZ or LAB values of the image?

When we use picasa we can notice a spectrum of the image that is displayed in the window and it shows various details about the image.. Also if the image is a single color image it can be...

07 August 2013 9,097 11 View

What is optical band gap and electrical band gap?

Is there any way to find out optical band gap and electrical band gap using simple instruments like UV-Vis spectrophotometer? What are the parameters that influence the band gap in a metal thin...

06 July 2013 2,742 3 View

How to setup a film thickness measurement instrument based on Tolansky method?

Please share your experin=ence and knowledge in setting up an Interferometric thin film thickness measuring instrument. I want a detailed step by step protocol to follow. I googled for it but in...

06 July 2013 9,169 11 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View