We are using a system that has metal ions. We need to use a pH 3 or 4 buffer that does not contain organic acids, as they chelate the metals.
You can use Glycine - Hydrochloric Acid (0.1 M Glycine titrated with HCl to necessary pH)
Have you considered phosphoric acid/sodium phosphate?
For this pH range the best buffer capacity are:
- malic acid/sodium hydroxide
- citric acid/sodium dihidrogencitrate
- formic acid/sodium formate
- glycolic acid/sodium hydroxide
- sodium fluoride/hidroclhoric acid
- phthalic acid/sodium hydroxide
Attach the chapter book dedicated to buffer preparation, but I recommend use the "BufferMaker" Software. Regards
I'm working on a project involving gene transcription and translation rates in Klebsiella pneumoniae and Acinetobacter baumannii. Is there a codon optimization tool for non-model organisms? Most...
09 October 2019 384 2 View
Anyone know of good BM3 p450 substrates that have fluorescent or colored products? I've used indole and coumarin, but both only work for one enzyme variant each.
07 August 2016 9,279 1 View
synonyms: Methyl phenyl carbinol, styrene alcohol, alpha-methyl benzyl alcohol. CAS: 98-85-1 Specifically, I need the extinction coefficient at 230 nm, perhaps for any peaks and troughs in the...
09 October 2014 6,687 1 View
I asked some suppliers for quotes, but they may be slow in responding, and figured I could get details here sooner. Minimum 50,000x magnification, but preferably 100,000x.
09 October 2014 6,351 2 View
I am collaborating with another lab that was supposed to prepare a nutrient rich media for me, but they have been unresponsive. I have some E. coli and some Lima Bean Agar plates laying around,...
08 September 2014 5,604 4 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View