Is there a codon optimization tool for gene expression in non-model organisms?

I'm working on a project involving gene transcription and translation rates in Klebsiella pneumoniae and Acinetobacter baumannii. Is there a codon optimization tool for non-model organisms? Most...

09 October 2019 205 2 View

Is there a buffer that can operate in the 3-5 pH range that does not contain organic acids?

We are using a system that has metal ions. We need to use a pH 3 or 4 buffer that does not contain organic acids, as they chelate the metals.

06 July 2015 6,474 3 View

Does anyone have access to UV/vis spectra for 1-phenyl ethanol?

synonyms: Methyl phenyl carbinol, styrene alcohol, alpha-methyl benzyl alcohol. CAS: 98-85-1 Specifically, I need the extinction coefficient at 230 nm, perhaps for any peaks and troughs in the...

09 October 2014 6,547 1 View

Does anyone know around how much a benchtop SEM costs?

09 October 2014 6,202 2 View

Does anyone know if E. coli can be grown on Lima Bean Agar?

I am collaborating with another lab that was supposed to prepare a nutrient rich media for me, but they have been unresponsive. I have some E. coli and some Lima Bean Agar plates laying around,...

08 September 2014 5,497 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View