Anyone know of good BM3 p450 substrates that have fluorescent or colored products? I've used indole and coumarin, but both only work for one enzyme variant each.
Here is a 2005 paper on fluorogenic alkoxyresorufin substrates:
http://www.ncbi.nlm.nih.gov/pubmed/15866539
I'm working on a project involving gene transcription and translation rates in Klebsiella pneumoniae and Acinetobacter baumannii. Is there a codon optimization tool for non-model organisms? Most...
09 October 2019 384 2 View
We are using a system that has metal ions. We need to use a pH 3 or 4 buffer that does not contain organic acids, as they chelate the metals.
06 July 2015 6,607 3 View
synonyms: Methyl phenyl carbinol, styrene alcohol, alpha-methyl benzyl alcohol. CAS: 98-85-1 Specifically, I need the extinction coefficient at 230 nm, perhaps for any peaks and troughs in the...
09 October 2014 6,687 1 View
I asked some suppliers for quotes, but they may be slow in responding, and figured I could get details here sooner. Minimum 50,000x magnification, but preferably 100,000x.
09 October 2014 6,351 2 View
I am collaborating with another lab that was supposed to prepare a nutrient rich media for me, but they have been unresponsive. I have some E. coli and some Lima Bean Agar plates laying around,...
08 September 2014 5,604 4 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View