Is their any differences between ECL western blotting apparatus and gel band fluorescent detection apparatus?

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Diltuions for a Western blot?

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(western blot) can methanol damage my antibody signal?

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Dominique Liger

Hi there,

ECL is about luminescence only (light emission from a chemical reaction) whereas fluorescence comes from light excitation at specific wavelength. Detection/quantification of both is made through image analysis of emitted light. So I think fluorescence detection apparatus may be use for ECL (in fact it is actually used as a dark room as no excitation illumination is needed to observe light emission)