Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Which root in this tap root system, in the picture attached, should be used for root sectioning in order to study the root anatomical details?

There are numerous roots in this tap root system. Kindly suggest or share a research paper where I can clarify the doubts as to which root should be chosen for sectioning and for further root...

14 February 2021 1,816 3 View

Hi, i am working on PARP inhibitors poorly water soluble but soluble in toxic solvents acetonitrile / DMSO, please suggest nontoxic solvents ?

Drug is poorly water soluble and soluble in solvents like acetonitile , DMSO, mixture of methanol and water but they are toxic. kindly suggest any safe solvent.

18 September 2020 5,128 4 View

How do I conclude if my gene has low expression based on the results obtained?

Hello all, I am working on genes affected in cholesterol metabolism in patients with Coronary Artery Disease and PCSK9 is one such target gene in my study. I have managed to obtain stable results...

13 June 2020 7,074 9 View

What is the institute/organization that provides training for hydroponics development ?

I also want to know the organization working on development of nutrient solution for hydroponics and the organization providing training on it.

02 June 2020 8,901 4 View

What method can I use to analyse the language on Social media?

I'm doing a comparative study on social media language used by native and non-native speakers with special reference to Instagram. I am planning on using Discourse analysis. What is your take on...

18 April 2020 300 25 View

In biosorption process (Lantana camara leaves as biosorbent), why do we get a precipitate after putting the filtrate on hot plate for agitation?

Usually, we get a clear filtrate after performing batch experiments; same is with Lantana indica leaves as biosorbent. But, after filtration we observe dark precipitation in this case. Is it due...

03 April 2020 9,406 1 View

Are there reserach papers on detecting whole multivariate time-series as anomalous from a set of multivariate time-series?

Consider a scenario with Dataset D as {T1, T2, ..., Tn} and Ti is a multivariate time-series of length mi as {X1, X2, ..., Xmi}. Here each record of the time-series Xi is a vector of attribute...

05 February 2020 967 2 View

Procuring AZ81 alloy ?

Dear Ma'am, I am interested in working with Mg-alloy since it is highly flammable, I am not able to procure the material nor create the composition of AZ series in the foundry. kindly help me...

12 January 2020 2,365 3 View

Which organic solvent is best for phytochemical extraction of plant leaf material??

Describe selection of best organic solvent for extraction of phyto chemicals from plant leaf.

10 November 2019 2,389 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View