Is the RLM-RACE suitable for detection of miRNA targets in genes whose sequence is well known?

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Michael Rak

No. RLM-Race is great for finding the exact 5' cap and for mapping splice variants, but you don't need to do full RLM-RACE to determine the targeting of an miRNA.

Ajay Saini

Hi Lucia'

5' RLM- RACE is definitely a good experimental to identify the cleavage site in the transcripts where the miRNA binds...

bye