It seems the dual virus-based monosynaptic tracing, i.e.G-TVA plus EnvA-pseudotyped deltaG RABV, is a widely used technique for seeking presynaptic partners of host cells. See (Bergami et al., 2015, Neuron 85, 710–717) and (Garcia et al., 2014, Dev Cell 30, 645-659). Is there any possibility of leakage?
Is there any comparison with GRASP technique? (http://www.nature.com/nprot/journal/v9/n10/full/nprot.2014.166.html)
On the base of my experience, the Rabies virus works very well and is a specific technique. It is rare to have a possibility of leakage.
The RABV infected cells are alive 2-3 weeks after the infection and leakage is not an issue since budding occurs at the synapse. The picture you'll get with mGRASP will be different (seeing synapses) and you'd probably use it to see if region A and B (or cell type A and B) are in general connected, but not to see which cells exactly project to your cell of interest.
So it depends a bit on the question at hand, which method to chose or if its necessary to combine both.
thanks, it seems toxicity is an big issue for RABV, since in most cases the starter cells will die soon, and the infected cells keep dying. we will see if a reduced toxicity RABV is available.
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