I collected the pancreas from rats and stored them in OCT at -80 degrees C. I intend to treat the tissue cuts by immunosuppressors like FK506 or rapamycin for IHC analysis.
Thank you for your responses.
I am not expecting that you are dealing with live tissue any more. If you want to cryopreserve pancreatic cancer tissue please refer to our work we published back in 2001 (see attachment). Hope this helps too. Claudio
Article Successful xenografting of cryopreserved primary pancreatic cancers
If you thaw now the frozen-down entire "normal" pancreatic gland, then the proteases will chew up the tissue. The immunosuppressors will not work on tissue slices - unless you cut "thick/organotypic" ones Even then, the out one is unclear to be optimistic.
Thank you so much for your time. I appreciate the answers. The dilemma is that in our lab my colleagues managed to treat OST-frozen aorta. They had even changes in specific marker staining between different conditions. Is this a "real" tissue answer? But because of the reasons enunciated by you I have my doubts. Our lab is specialized in cellular senescence. therefore, the immunosuppressor treatment is to observe their effect on cell response.
Thank you again!
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