If two miRNAs differ in their seed sequence for one nucleotide in the 7 position, do they share the same set of targets?
I agree with Darrel that it is difficult to predict, however, nowadays, we have several prediction tools like RNAhybrid, Targetscan, PITA etc.... which can be very helpful in the prediction of targets. Previously, it was thought that only canonical sequence binding is very important but recent studies have revealed that non-canonical binding also plays a very important role and are truly functional.
For the second part of your question, as different miRNAs can control the same target and similarly one miRNA can target different mRNAs, so there are chances that they might share the same target even with one nucleotide difference.
I agree with Darrel that it is difficult to predict, however, nowadays, we have several prediction tools like RNAhybrid, Targetscan, PITA etc.... which can be very helpful in the prediction of targets. Previously, it was thought that only canonical sequence binding is very important but recent studies have revealed that non-canonical binding also plays a very important role and are truly functional.
For the second part of your question, as different miRNAs can control the same target and similarly one miRNA can target different mRNAs, so there are chances that they might share the same target even with one nucleotide difference.
Hi Matilde
In principle the answer is YES... they will share the same family of targets, however their binding affinities to the target could be different, and consequently the expected regultory effect.
Best
Paco
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