It's worked fine for me in the past. You could also just add 500 mM imidazole to the washed beads then centrifuge to remove the beads. Make sure that you don't get any beads in you SDS-PAGE lane as it will ruin the gel. Hope this helps and good luck!

Hi Sean, I thought of adding imidazole too but will it interfere with the western blot result? Thanks. 

It hasn't been an issue when I've done it. It is on a multi-lane gel. You could always split your sample and try it a few different ways on the same gel.