Recommendation on substrates for 2D neuronal migration assay?

Hi does anyone have any recommendations on what type of substrates I can try for 2D neuronal migration. I've seen the use of cover slips coated with collagen and fibronectin, and also the use of...

09 October 2016 6,989 0 View

How are conditions for bacterial cell sonication determined?

Hi guys, I am currently optimising the expression of a protein that is found predominantly in the pellet. However, I am unsure how the sonication condition can be adjusted such that I have a...

11 December 2014 2,018 8 View

A query on protein purification and refolding from insoluble fraction

Hi, I recently purified an eukaryotic protein from BL21 cells. Upon examining the fractions, it seems that this protein is found predominantly in the insoluble pool. As such, I need some advice on...

09 October 2014 7,184 8 View

What is the purpose of Tetrabutylammonium bromide in HPLC?

I chanced upon a HPLC protocol that uses acetonitrile, Potassium phosphate and tetrabutylammonium bromide as the solvent for reverse phase chromatography. I believe potassium phosphate is most...

08 September 2014 4,221 14 View

Can IPTG induced cells be kept in the pellet form at minus 20 degree Celsius for prolonged period before cell lysis?

I heard that once the supernatant of cell culture is removed, the pellet can be kept in minus 20 fridge. However, I wonder if this can be done for long period of time. Also, please suggest if...

08 September 2014 7,356 7 View

What are the recommended ways to preserve proteins that are to be subjected to downstream activity tests?

Hi everyone, may i know how proteins can be preserved upon purification? I need these proteins for downstream activity tests but as I will not need them right away, I need some solutions to...

08 September 2014 8,384 7 View

What considerations should I make over the types of salt present in buffers?

Hello guys, may I know what are the considerations one should have when it comes to the selection of buffers for protein purification? I learnt that optimal pH range and components such as...

07 August 2014 5,430 5 View

Any advice on reduced yield of purified protein upon dialysis (Pierce slide A lyzer) and concentrating (Amicon 10K)?

Hello guys, I have recently purified a his-tagged protein via Ni beads. Based on a BSA standard, it was estimated that the eluted protein concentration was about 1 microgram per microlitre in 100...

07 August 2014 2,141 10 View

Is the loading buffer (lamelli) sufficient for removing proteins that are pulled down by Nickel beads for western blot?

07 August 2014 4,427 3 View

How can I estimate a protein isoelectric point and its implication on purification?

Hi guys, I would like to know if there is any recommended websites that I can rely on for isoelectric point estimation? I used the one by expasy and it seems that the value deviates by at least...

06 July 2014 3,038 20 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View