Adam Wei Jian Soh

11 Questions 29 Answers 0 Followers

Questions related from Adam Wei Jian Soh

Recommendation on substrates for 2D neuronal migration assay?

Hi does anyone have any recommendations on what type of substrates I can try for 2D neuronal migration. I've seen the use of cover slips coated with collagen and fibronectin, and also the use of...

10 October 2016 7,052 0 View

How do I generate a generic protein motif?

Hi everyone, I am currently working on a project that requires me to generate a generic motif that I can blast against my database of interest. Could someone guide me on how this can be done?...

01 January 2015 9,098 3 View

How are conditions for bacterial cell sonication determined?

Hi guys, I am currently optimising the expression of a protein that is found predominantly in the pellet. However, I am unsure how the sonication condition can be adjusted such that I have a...

12 December 2014 2,150 8 View

A query on protein purification and refolding from insoluble fraction

Hi, I recently purified an eukaryotic protein from BL21 cells. Upon examining the fractions, it seems that this protein is found predominantly in the insoluble pool. As such, I need some advice on...

10 October 2014 7,254 8 View

Can IPTG induced cells be kept in the pellet form at minus 20 degree Celsius for prolonged period before cell lysis?

I heard that once the supernatant of cell culture is removed, the pellet can be kept in minus 20 fridge. However, I wonder if this can be done for long period of time. Also, please suggest if...

09 September 2014 7,454 7 View

What is the purpose of Tetrabutylammonium bromide in HPLC?

I chanced upon a HPLC protocol that uses acetonitrile, Potassium phosphate and tetrabutylammonium bromide as the solvent for reverse phase chromatography. I believe potassium phosphate is most...

09 September 2014 4,365 14 View

What are the recommended ways to preserve proteins that are to be subjected to downstream activity tests?

Hi everyone, may i know how proteins can be preserved upon purification? I need these proteins for downstream activity tests but as I will not need them right away, I need some solutions to...

09 September 2014 8,448 7 View

Is the loading buffer (lamelli) sufficient for removing proteins that are pulled down by Nickel beads for western blot?

If not, are there any alternative methods to prepare samples? Thanks.

08 August 2014 4,497 3 View

What considerations should I make over the types of salt present in buffers?

Hello guys, may I know what are the considerations one should have when it comes to the selection of buffers for protein purification? I learnt that optimal pH range and components such as...

08 August 2014 5,490 5 View

Any advice on reduced yield of purified protein upon dialysis (Pierce slide A lyzer) and concentrating (Amicon 10K)?

Hello guys, I have recently purified a his-tagged protein via Ni beads. Based on a BSA standard, it was estimated that the eluted protein concentration was about 1 microgram per microlitre in 100...

08 August 2014 2,206 10 View

How can I estimate a protein isoelectric point and its implication on purification?

Hi guys, I would like to know if there is any recommended websites that I can rely on for isoelectric point estimation? I used the one by expasy and it seems that the value deviates by at least...

07 July 2014 3,099 20 View