Is the extinction coefficient of duplex DNA is sum of extinction coefficient of each single strand DNA? or something else ?

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Adam B Shapiro

The answer is no. The extinction coefficient of double-stranded DNA is less than the sum of the extinction coefficients of the individual strands. This property is known as hypochromicity. It is caused by the stacking of the bases in dsDNA.

Prabesh Gyawali

Is there any way to determine the extinction coefficient than ?

Have a look at this:

https://www.atdbio.com/content/1/Ultraviolet-absorbance-of-oligonucleotides#Hypochromicity-and-double-stranded-DNA

Arvind Swamy

Hey, Have a look at this link.. might be of some help ..

https://www.promega.com/-/media/files/resources/application-notes/pathlength/calculating-nucleic-acid-or-protein-concentration-using-the-glomax-multi-microplate-instrument.pdf?la=en