How stable are TCEP-reduced oligos (5'S-S modifier) once purified?

Hi there, I attempted to conjugate some short (~20bp) oligo probes using a commercial kit, but I believe the conjugation failed due to the use of an incorrect filter size. I still have the...

12 August 2019 7,390 1 View

How to measure hydrogen gas in high concentrations without using GC or RGA?

I'm trying to find a lower-cost method of measuring hydrogen gas concentrations in the region of 70-100%. Ideally some sort of sensor that can be used in a oxygen environment (which I am led to...

16 December 2018 471 2 View

Are there any tools available for primer design using a degenerate DNA template?

I'm currently trying to design a set of degenerate primers to amplify an identified conserved region among our target proteins, but I can't find any tool that accepts non-ATCGN degenerate...

26 October 2017 5,828 2 View

Is there a standard etiquette to utilizing surveys built by other researchers, especially if you modify them?

I am building a social support survey utilizing three established surveys, and adding three of my own questions. I am tailoring this survey to examine a specific population of college students...

09 December 2015 2,206 4 View

What are a few good strengths-based assessment tools?

10 November 2015 6,899 6 View

Does the qPCR reaction volume overall effect on CT?

I am struggling right now with my one-step qPCR for my two GOIs. They seem to amplify at a very high CT, and this leads to an impossibility in getting a decent standard curve. At full...

23 June 2014 8,785 8 View

Can you help me with Amplification in NTC (only for some primers) RT-qPCR?

Okay, I know the obvious answer is primer dimers, but I looked at the melt curves, and I am seeing the expected Tm. But I have been getting amplification in my NTC controls in my qPCR. The Cts...

19 June 2014 1,028 2 View

Diluting RNA for one-step qPCR

I was struggling with the standard curves for my RT-qPCR, and my PI suggested trying one-step instead of 2-step. After diluting one of my RNA samples to generate a standard curve (started with...

03 June 2014 1,157 1 View

Can anyone offer advice on using qPCR to compare homolog expression?

Quick background: I want to compare the expression of two homologs in potato. The two genes are >80% identical, so designing primers has to be a tricky process. I have designed a few of my own,...

13 May 2014 4,353 7 View

Should I continue to use Betaine in my master mix?

I have been using Betaine for a while now in my master mix, but I am beginning to question its effectiveness. I know it supposedly serves a purpose to improve target specificity, but there are...

02 April 2014 4,438 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

absorption= 1-mag(S(FP1:1,FP1:1)*S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)*S(FP2:1,FP1:1)) is it correct for absorption ?

hi everyone I am simulating a unit cell structure in HFSS using Flouquet port having two modes for absorption I have used this formula:- absorption=...

17 February 2021 9,178 2 View

How can I calculate the magnetic force applied from a magnet (no electrism)?

I would like to calculate the magnetic force on a Fe304 particle which is into a liquid solution . The magnet is above the solution , and there is no electricity. I found this formula : F = (V...

13 February 2021 4,770 3 View

What is the best way to do symmetry in ANSYS Fluent for flow between two concentric cylinders ?

I am working on Taylor Couette flow between concentric cylinders and the results are correct for the full case. however, I wanted to do symmetry case to improve the mesh. I have tried periodic...

11 February 2021 2,193 6 View

What is the ideal value of UACI for image encryption scheme?

UACI - Unified Averaged Changed Intensity between cipher images. Why some papers refer ideal value to be 33.33 and some refer it to be 33.46? Which is the correct value? (In reference to image...

11 February 2021 9,562 2 View